xcl-1 ipscs  (XCell Science)

Journal: Cell reports

Article Title: Opposing roles of p38α-mediated phosphorylation and PRMT1-mediated arginine methylation in driving TDP-43 proteinopathy

doi: 10.1016/j.celrep.2024.115205

Figure Lengend Snippet:

Article Snippet: Human male XCL-1 iPSCs , XCell Science , XCL-1 iPSC.

Techniques: Virus, Recombinant, Protease Inhibitor, Membrane, Western Blot, Stripping, Modification, Transfection, Fractionation, Cell Culture, Lysis, Magnetic Beads, Electron Microscopy, Staining, Bicinchoninic Acid Protein Assay, Kinase Assay, LDH Cytotoxicity Assay, Mutagenesis, CyQUANT Assay, Sequencing, Negative Control, DNA Sequencing, Plasmid Preparation, Software, Imaging

Journal: Cell reports

Article Title: Opposing roles of p38α-mediated phosphorylation and PRMT1-mediated arginine methylation in driving TDP-43 proteinopathy

doi: 10.1016/j.celrep.2024.115205

Figure Lengend Snippet:

Article Snippet: On Day 34 of differentiation iMNs derived from human male XCL-1 iPSCs (XCell Science) were subjected to proteotoxic stress by treatment with 0.5μM MG-132 (Millipore Sigma) plus 0.1% DMSO or 1μM VX-745 for 24 h. On day 35 cells were harvested and subjecyed to Sequential extraction of insoluble protein aggregates.

Techniques: Virus, Recombinant, Protease Inhibitor, Membrane, Western Blot, Stripping, Modification, Transfection, Fractionation, Cell Culture, Lysis, Magnetic Beads, Electron Microscopy, Staining, Bicinchoninic Acid Protein Assay, Kinase Assay, LDH Cytotoxicity Assay, Mutagenesis, CyQUANT Assay, Sequencing, Negative Control, DNA Sequencing, Plasmid Preparation, Software, Imaging